Protein_Domain

Part:BBa_K2124003:Design

Designed by: Brian Dempsey   Group: iGEM16_Lethbridge_HS   (2016-10-13)


Venom Serine Protease from Cerastes Cerastes - Thrombin analog - DsbC Disulfide shuffling


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 997
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This Serine Protease protein is very small and stabilized by several disulfide bonds. The final construct is secreted into the periplasmic space with DsbC, a protein that shuffles disfulfide bonds. DsbC will allow the Serine Protease to fold properly with correct disulfide bonding. This will give stable functional protein. The DsbC gene is contained as part of this construct.


Source

Both the DsbC and Venom Serine Protease are from genomic sequence.

References