Protein_Domain
Part:BBa_K2124003:Design
Designed by: Brian Dempsey Group: iGEM16_Lethbridge_HS (2016-10-13)
Venom Serine Protease from Cerastes Cerastes - Thrombin analog - DsbC Disulfide shuffling
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 997
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This Serine Protease protein is very small and stabilized by several disulfide bonds. The final construct is secreted into the periplasmic space with DsbC, a protein that shuffles disfulfide bonds. DsbC will allow the Serine Protease to fold properly with correct disulfide bonding. This will give stable functional protein. The DsbC gene is contained as part of this construct.
Source
Both the DsbC and Venom Serine Protease are from genomic sequence.